Staining of Tissue Sections for Electron Microscopy with Heavy Metals
نویسنده
چکیده
Descriptions of three heavy metal stains and methods of application to tissue sections for electron microscopy are presented. Lead hydroxide stains rather selectively two types of particles in liver: those associated with the endoplasmic reticulum and containing ribonucleic acid and other somewhat larger particles. Barium hydroxide emphasizes certain bodies within vesicles of the Golgi region of hepatic cells. Alkalized lead acetate is useful as a general stain, as are also lead and barium hydroxides.
منابع مشابه
Staining of Tissue Sections for Electron Microscopy with Heavy Metals II. Application of Solutions Containing Lead and Barium*
Descriptions of three heavy metal stains and methods of application to tissue sections for electron microscopy are presented. Lead hydroxide stains rather selectively two types of particles in liver: those associated with the endoplasmic reticulum and containing ribonucleic acid and other somewhat larger particles. Barium hydroxide emphasizes certain bodies within vesicles of the Golgi region o...
متن کاملThe Use of Mercuric Bromphenol Blue as a Stain for Electron Microscopy
The search for suitable staining methods for use in electron microscopy has led to many and diverse attempts to increase the opacity to electrons of different structures in tissue sections. The most obvious approach has been the attempt to bind heavy metals to specific sites, either to increase the general contrast or to identify the chemical nature of the structures so stained. Other than the ...
متن کاملLight and electron microscopic observations of glycogen in semi-thin sections employing GMA, Quetol 523 and methylmethacrylate.
The use of improved GMA-Quetol 523 or GMA-Quetol 523 methyl methacrylate (MMA) mixture as an infiltration medium for the histochemical demonstration of glycogen has been devised to facilitate embedding, sectioning and staining. An improved method of infiltration uses such mixtures with a double weight of QCU-1 as a catalyst. Since the double weight of catalyst prevents interference by bleeding ...
متن کاملHigh Efficient Nanocomposite for Removal of Heavy Metals (Hg2+ and Pb2+) from Aqueous Solution
In current work, CdS/black carbon nanocomposites were successfully synthesized with the aid of chestnut and cadmium nitrate as the starting reagents. Besides, the effects of preparation parameters such as reaction time, and precursor concentration on the morphology of products and removal of heavy metals (Hg+2, Pb+2) were studied by scanning electron microscopy images and batch adsorption mode....
متن کاملHot Alcoholic Phosphotungstic Acid and Uranyl Acetate as Routine Stains for Thick and Thin Sections
One of the potential advantages of high-voltage electron microscopes is that their greater penetrating power allows the use of thick sections (0.22 lt) to study three-dimensional cytoarchitecture . So far, this advantage has been limited because conventional methods of staining only allow the penetration of heavy metal solutions into sections to a depth of a few hundred Angstroms . For example,...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- The Journal of Biophysical and Biochemical Cytology
دوره 4 شماره
صفحات -
تاریخ انتشار 1958